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EZHS100B

Human S100B ELISA Kit

Name: Human S100B ELISA Kit
Brand: MM
Price: 898 USD

quantifies S100B in 50 μL cerebrospinal fluid, serum or plasma

Sinónimos:

Protein S100B, S-100 protein beta chain, S100 calcium-binding protein B

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Talla/SKU	Disponibilidad	Precio
96 wells	Comprobar disponibilidad del carrito	$898.00

Acerca de este artículo

UNSPSC Code:

12161503

eCl@ss:

32161000

NACRES:

NA.84

Input:

sample type cerebrospinal fluid (CSF)
sample type plasma (K2 EDTA)
sample type serum

Species reactivity:

human

Storage temp.:

2-8°C

Shipped in:

wet ice

$898.00

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Propiedades

Nombre del producto

Human S100B ELISA, EZHS100B-33K, This Human S100B ELISA kit is to be used for the quantification of human S100B in cerebrospinal fluid, serum & plasma.

Quality Segment

200

species reactivity

human

packaging

kit of 1 × 96 wells

parameter

50 μL sample volume (4hr assay)

technique(s)

ELISA: suitable

input

sample type cerebrospinal fluid (CSF)
sample type plasma (K2 EDTA)
sample type serum

UniProt accession no.

P04271

application(s)

research use

assay range

accuracy: 93%
(CSF), accuracy: 94%
(serum), sensitivity: 2.7 pg/mL, standard curve range: 2.7-2000 pg/mL, inter-assay cv: <4%
intra-assay cv: <4%

detection method

colorimetric (450nm)

shipped in

wet ice

storage temp.

2-8°C

Descripción

General description

EMD Millipore’s Human S100B ELISA kit is to be used for the quantification of human S100B in cerebrospinal fluid, serum and plasma. This kit has not been tested for cell culture supernatants.

S100B is a calcium-binding protein produced mainly by astrocytes in the central nervous system and has been implicated in the development and maintenance of the nervous system. Although the mechanism of S100B secretion is unknown, it appears to be affected by oxidative stress. At nanomolar concentrations (normal levels), S100B is able to protect neurons against glutamate toxicity. Glial damage or astrocytic reactions to neural injury (reactive astrogliosis) causes an increase in S100B to micromolar concentrations. High levels of S100B will further increase neuronal damage. High levels of S100B are considered a biomarker that could indicate damage or dysfunction of CNS.

Application

Research Category
Neuroscience

This is a same day assay

Used to detect/quantify: S100B

Biochem/physiol Actions

The antibody pair used in this assay measures Human S100B and has no cross-reactivity with S100A1, S100A6 and S100A13.

This assay detects S100B in multiple species, including: bovine, hamster, horse, monkey, mouse, pig, rabbit, and rat.

Other Notes

Please contact Technical Service for linearity of dilution.

Disclaimer

For research use only. Not for use in diagnostic procedures.

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Este artículo	ABN59	RAB1673	RAB1676
Millipore EZHS100B Human S100B ELISA, EZHS100B-33K	Sigma-Aldrich ABN59 Anti-S100B Antibody	Sigma-Aldrich RAB1673 Human S100 A1 ELISA	Sigma-Aldrich RAB1676 Human S100 A7 ELISA
description This Human S100B ELISA kit is to be used for the quantification of human S100B in cerebrospinal fluid, serum & plasma.	description from rabbit, purified by affinity chromatography	description for serum, plasma and cell culture supernatants	description for serum, plasma and cell culture supernatants
species reactivity human	species reactivity human	species reactivity human	species reactivity human
assay range sensitivity: 2.7 pg/mL, standard curve range: 2.7-2000 pg/mL, accuracy: 94% (serum), inter-assay cv: <4% intra-assay cv: <4%, accuracy: 93% (CSF)	assay range -	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 0.82 ng/mL standard curve range: 0.819-200 ng/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 24 pg/mL standard curve range: 24.58-6000 pg/mL
input sample type cerebrospinal fluid (CSF) sample type plasma (K2 EDTA) sample type serum	input -	input sample type plasma sample type serum sample type cell culture supernatant(s)	input sample type cell culture supernatant(s) sample type serum sample type plasma
Quality Level 200	Quality Level 300, 100	Quality Level -	Quality Level -
technique(s) ELISA: suitable	technique(s) western blot: suitable, immunohistochemistry: suitable (paraffin)	technique(s) ELISA: suitable	technique(s) ELISA: suitable
detection method colorimetric (450nm)	detection method -	detection method colorimetric	detection method colorimetric

Pictogramas

GHS06,GHS05,GHS09

Palabra de advertencia

Danger

Códigos de peligro

H290,H302 + H332,H311,H317,H411

Indicaciones de precaución

P234 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1

Clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

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Questions

1–2 of 2 Questions

Anonymous

2 years ago

Is the EZHS100B-33K ELISA assay compatible with human saliva samples?

1 answer
1. Technical Support
  
  2 years ago
  
  The kits have not been validated for use with saliva; however, some guidelines are available for processing saliva samples:
  
  1. Add protease inhibitor cocktail (Product P2714) at a 1:500 ratio to the saliva.
  2. Centrifuge the mixture at 10,000 rpm for 10 minutes.
  3. Dilute the supernatant in a 1:2 ratio with Assay Buffer before to setting up the assay.
  4. Run assay with Assay Buffer as the matrix for the standard curve.
  5. Multiply by 2 for the final result.
  
  It should be noted that there are no references available for the S100B ELISA kit being used with saliva samples.
  
  Helpful?
Anonymous

2 years ago

Which antibodies have been used for the Human S100B ELISA kit (EZHS100B-33K)?

1 answer
1. Technical Support
  
  2 years ago
  
  The origins of the antibodies utilized in the kit are considered proprietary. Nevertheless, some information about the antibodies can be shared. The capture antibody is a monoclonal mouse anti-S100B, with bovine brain S100B used as the immunogen. The detection antibody is a polyclonal rabbit anti-S100, and the immunogen for this is also sourced from bovine brain S100.
  
  Helpful?

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