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CBL221

Sigma-Aldrich

Anti-RNA Polymerase II Antibody, clone ARNA-3

ascites fluid, clone ARNA-3, Chemicon®

Sinónimos:

DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B1

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100 μL
$513.00

$513.00

Fecha estimada de envío25 de mayo de 2025

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100 μL
$513.00

About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

$513.00

Fecha estimada de envío25 de mayo de 2025

origen biológico

mouse

Nivel de calidad

100

forma del anticuerpo

ascites fluid

tipo de anticuerpo

primary antibodies

clon

ARNA-3, monoclonal

reactividad de especies

kangaroo, human, Drosophila

fabricante / nombre comercial

Chemicon®

técnicas

immunohistochemistry: suitable
western blot: suitable

isotipo

IgG1

Nº de acceso NCBI

NP_511124.1

Nº de acceso UniProt

P04052

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... POLR2A(5430)

Descripción general

DNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; UniProt P04052; also known as DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B1) is encoded by the RpII215 (also known as CG1554) gene (Gene ID 32100) in drosophila melanogaster (fruit fly). RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

Especificidad

ARNA-3 is excellent for detection of transcription activity, e.g. puffs in polytene chromosomes. Reacts with RNA polymerase II in biological materials. Reactive polypeptides after SDS-PAGE: 200 kDa band of nonphosphorylated and 240 kDa band of phosphorylated Pol IIA.
Broad species cross-reactivity expected due to sequence homology.

Inmunógeno

Epitope: Amino acids 794-822
Purified Drosophila RNA polymerase II

Aplicación

Detect RNA Polymerase II with Anti-RNA Polymerase II Antibody, clone ARNA-3 (Mouse Monoclonal Antibody), that has been shown to work in WB & IHC.
Western blot
Immunohistochemistry: 1:100; Frozen tissue; fixation recommendation - incubate tissue with 2% paraformaldehyde in PBS for 10 min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min. Incubate with antibody one hour.
Optimal working dilutions must be determined by the end user.

Forma física

Supplied as hybridoma culture supernatant concentrate containing 0.09% sodium azide.

Nota de análisis

Control
Positive for drosophila, kangaroo kidney epithelial cell line PtK2, and human HeLa and HEp-2 cell lines.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Tim Patrick Kaminski et al.
Molecular biology of the cell, 25(14), 2190-2198 (2014-05-23)
Regulation of RNA polymerase II (RNAPII) during transcription is essential for controlling gene expression. Here we report that the transcriptional activity of RNAPII at the Balbiani ring 2.1 gene could be halted during stable elongation in salivary gland cells of
Shih-Ying Tsai et al.
Epigenetics & chromatin, 9, 32-32 (2016-07-29)
Genome-wide studies in higher eukaryotes have revealed the presence of paused RNA polymerase II (RNA-Pol) at about 30-50 bp downstream of the transcription start site of genes involved in developmental control, cell proliferation and intercellular signaling. Promoter-proximal pausing is believed to
Roberta Dollinger et al.
The Journal of biological chemistry, 299(9), 105106-105106 (2023-07-31)
Promoter proximal pausing of RNA polymerase II (Pol II) is a critical transcriptional regulatory mechanism in metazoans that requires the transcription factor DRB sensitivity-inducing factor (DSIF) and the inhibitory negative elongation factor (NELF). DSIF, composed of Spt4 and Spt5, establishes

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