ABS1491

Anti-Frizzled-2

from rabbit, purified by affinity chromatography

• Sinónimos: Fz-2, hFz2, FzE2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): mouse (based on 100% sequence homology)
• envase: antibody small pack of 25 μL
• técnicas: western blot: suitable
• Nº de acceso UniProt: Q14332
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... FZD2(2535)

Descripción

Descripción general

Frizzled-2 (UniProt: Q14332; also known as Fz-2, hFz2, FzE2) is encoded by the FZD2 gene (Gene ID: 2535) in human. Frizzled-2 is a multi-pass membrane protein that is widely expressed in adult tissues. In fetus, higher levels are expressed in the brain, lung, and kidney and lower levels are observed in liver. Frizzled serves as a receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. In the presence of Wnt the Frizzled receptor is activated, which along with low-density lipoprotein receptor-related proteins (LRP) 5 and 6 stabilizes beta-catenin. The formation of Wnt-Frizzled-LRP6 complex, together with the recruitment of the scaffolding protein Dishevelled (Dvl), results in LRP6 phosphorylation and activation and the recruitment of the Axin complex to the receptors. This leads to the inhibition of Axin-mediated beta-catenin phosphorylation, which stabilizes beta-catenin. Frizzled-2 is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off in the mature form. Its Fz domain is localized to amino acids 34-153 and this domain is involved in its binding with Wnt ligands. Frizzled-2 can be ubiquitinated by RING-type E3 ubiquitin transferase ZNRF3 leading to its proteasomal degradation. Abnormalities in Wnt signaling are reported to promote birth defects, degenerative diseases, and cancer.

Especificidad

This rabbit polyclonal antibody specifically detects human Frizzled -2. It targets an epitope with in 13 amino acids from the extracellular domain.

Inmunógeno

Epitope: unknown

KLH-conjugated linear peptide corresponding to 13 amino acids from the N-terminal half of human Frizzled-2.

Aplicación

Detects Frizzled-2 using this rabbit polyclonal Anti-Frizzled-2 Antibody, Cat. No. ABS1491, tested for use in Western Blotting.

Research Category
Signaling

Calidad

Evaluated by Western Blotting in CACO-2 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Frizzled-2 in CACO-2 cell lysate.

Descripción de destino

~60 kDa observed; 63.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.