ABS1013

Anti-MTHFR Antibody

serum, from rabbit

• Sinónimos: Methylenetetrahydrofolate reductase, MTHFR
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: serum
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: human, mouse, porcine
• técnicas: western blot: suitable
• Nº de acceso NCBI: NP_005948
• Nº de acceso UniProt: P42898
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MTHFR(4524)

Descripción

Descripción general

Methylenetetrahydrofolate reductase or 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20; UniProt P42898) is encoded by the MTHFR gene (Gene ID 4524) in human. MTHFR catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, the predominant circulatory form of folate and carbon donor for the re-methylation of homocysteine to methionine. Defects in MTHFR cause plasma homocysteine elevation or hperhomocysteinaemia, a risk factor for cerebrovascular, peripheral vascular, and coronary heart disease. Multiple transcription start sites and alternative splicing in the MTHFR gene result in two enzyme isoforms.

Especificidad

Reacts with both isoforms (PMID 12370778).

Inmunógeno

Recombinant protein corresponding to human MTHFR.

Aplicación

Detect MTHFR using this Anti-MTHFR Antibody validated for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Western Blotting Analysis: A representative lot detected MTHFR in mice plasma, kidney and brain (Chew, T.W., et al. (2011). J. Nutr. 141(8):1475-1481.).
Western Blotting Analysis: A representative lot detected MTHFR in porcine liver, human lymphocytes, human fetal kidney, human adult kidney, cells transfected with MTHFR (Rozen, R. U.S. Patent 6,833,243 B2, December 21, 2004).
Western Blotting Analysis: A representative lot detected MTHFR in HepG2, mouse liver, and RAW264.7 (Mikael, L.G., and Rozen, R. (2008). Cardiovasc. Res. 80(1):151-158).
Western Blotting Analysis: A representative lot detected recombinant MTHFR protein, as well as endogenous MTHFR in human kidney (both fetal & adult), fetal human lung, and adult human aorta (Tran, P., et al. (2002). Mamm. Genome. 13(9):483-492).

Calidad

Evaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected MTHFR in 10 µg of RAW264.7 cell lysate.

Descripción de destino

~70/77 kDa observed. Uncharacterized band(s) may appear in some lysates.

Forma física

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1