ABN495

Anti-DARC Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: DARC Antibody, Duffy antigen, Fy glycoprotein, GpFy, Cell adhesion molecule 3, CADM3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse
• species reactivity (predicted by homology): human (immunogen homology)
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• UniProt accession no.: Q16570
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ACKR1(2532)

Descripción

General description

Duffy antigen/chemokine receptor (DARC), also known as Fy glycoprotein (GpFy), is a multi-pass membrane protein belonging to the G-protein coupled receptor Duffy family. DARC binds, with high affinity, several chemokines without transducing any signal, suggesting it may modulate the signals normally induced by these chemokines. DARC is found in adult kidney, adult spleen, bone marrow, and fetal liver. It is expressed along postcapillary venules throughout the body, except in the adult liver.

Specificity

This antibody recognizes the C-terminus of DARC.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human DARC.

Application

Detect Duffy antigen/chemokine receptor using this rabbit polyclonal antibody, Anti-DARC Antibody validated for use in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected DARC in mouse brain tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected DARC in 15 µg of mouse brain tissue lysate.

Target description

~23 and 35 kDa observed. Uniprot describes two isoforms at ~35 and ~36 kDa

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable