ABN37
Anti-Homer1 Antibody
from rabbit, purified by affinity chromatography
Sinónimos:
Homer protein homolog 1, Homer-1, Homer1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
species reactivity (predicted by homology)
horse (based on 100% sequence homology), monkey (based on 100% sequence homology)
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HOMER1(9456)
General description
Homer protein homolog 1 (UniProt Q86YM7; also known as Homer-1, Homer1) is encoded by the HOMER1 (also known as SYN47) gene (Gene ID 9456) in human. Homer-1, -2, and -3 constitute a family of postsynaptic scaffold proteins that play a central role in calcium signaling. Homer members share a conserved N-terminal Ena/VASP homology 1 (EVH1) domain (a.a. 1-110 of Homer-1) that mediate signaling complexes assembly by interacting with PPXXFR proline-rich consensus sequences found in various other scaffolding and signal transduction molecules, including metabotropic glutamate receptors (mGluRs) and 1,4,5-trisphosphate receptors (IP3Rs). In addition to mediate group I mGluRs signaling via their EVH1 domain, Homer-1 spliced variants Vesl-1L, 1b, and 1d, but not Vesl-1M or Vesl-1S, also contain a C-terminal coiled-coil (a.a. 181-352) region that mediate self-multimerization. Homer-1 variants are associated with the etiology of many neurological diseases, such as chronic pain, mental retardation syndromes, Alzheimer′s disease (AD), Parkinson′s disease (PD), schizophrenia, drug-induced addiction, and traumatic brain injury (TBI). Homer1b/c-knockdown is reported to protect against chemically induced seizure in mice as a result of preventing mGluR5-mediated mTOR pathway activation.
Specificity
Broad species reactivity expected based on high sequence homology.
This rabbit polyclonal antibody targets an internal sequence within the N-terminal half of human Homer1 isoform 1b and mouse isoform Vesl-1L. Immunogen sequence appears near the C-terminus of human isoform 1h and mouse isoforms 1b, 1d, Vesl-1M, and Vesl-1S, but absent in human isoform 1e. Highly homologous sequence (~83%) is also found in the N-terminal half of rat isoform 1c (Vesl-1L) and near the C-terminus of rat isoforms 1a (Vesl) and 1b.
Immunogen
KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human Homer1 isoform 1b and near the C-terminus of isoform 1h.
Application
Anti-Homer1, Cat. No. ABN37, is a highly specific rabbit polyclonal antibody that targets Homer protein homolog 1 and has been tested in Immunohistochemistry and Western Blotting.
Immunohsitochemistry Analysis: A 1:500 dilution from a representative lot detected Homer1 in human and mouse cerebral cortex tissue sections.
Immunohsitochemistry Analysis: A representative lot detected significantly increased stratum radiatum and stratum oriens Homer1 immunoreactivity in floating brain sections from mice received AP-12 intraperitoneal injection (Jansone, B., et al. (2015). PLoS One. 10(6):e0127686).
Immunohsitochemistry Analysis: A representative lot detected significantly increased stratum radiatum and stratum oriens Homer1 immunoreactivity in floating brain sections from mice received AP-12 intraperitoneal injection (Jansone, B., et al. (2015). PLoS One. 10(6):e0127686).
Quality
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Homer1 in 10 µg of mouse brain tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Homer1 in 10 µg of mouse brain tissue lysate.
Target description
~47 kDa observed. 40.28/19.86 kDa (human isoform 1b/1h), 40.22/42.08/41.41/22.78/20.91 kDa (mouse isoform 1b/1d/Vesl-1L/Vesl-1M/Vesl-1S) calculated. Uncharacterized bands may be observed in some lysate(s).
Other Notes
Concentration: Please refer to lot specific datasheet.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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