ABN1346

Anti-SHIP1 Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1, hp51CN, Inositol polyphosphate-5-phosphatase of 145 kDa, p150Ship, SH2 domain-containing inositol 5′-phosphatase 1, SH2 domain-containing inositol phosphatase 1, SHIP-1, SHIP1, SIP-145
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• técnicas: immunohistochemistry: suitable; western blot: suitable
• Nº de acceso NCBI: NP_001017915
• Nº de acceso UniProt: Q92835
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... INPP5D(3635)

Descripción

Descripción general

Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 (EC 3.1.3.86; UniProt Q92835; also known as hp51CN, Inositol polyphosphate-5-phosphatase of 145 kDa, p150Ship, SH2 domain-containing inositol 5′-phosphatase 1, SH2 domain-containing inositol phosphatase 1, SHIP-1, SIP-145) is encoded by the INPP5D (also known as SHIP, SHIP1) gene (Gene ID 3635) in human. SHIP1 and SHIP2 are related phosphatases that play important roles in modulating cell signaling by regulating the phosphorylation state of phosphatidylinositol phosphates (PIPs). While SHIP1 and SHIP2 share a high degree of amino acid conservation, they differ significantly in their cellular expression and receptor recruitment. SHIP1 is primarily confined to cells of the hematopoietic lineage, although it is also expressed by embryonic and mesenchymal stem cells (ESCs and MSCs). SHIP2 is ubiquitously expressed in all cell and tissue types, with especially high levels found in the heart, skeletal muscle, and placenta. SHIP1 functions as s negative regulator in immunoreceptor signaling, in hematopoietic progenitor cell proliferation/survival, and as an inducer of cellular apoptosis. Humna SHIP1 consists of an N-terminal SH2 domain (a.a. 5-101) that mediates interactions with a large number of intracellular signaling proteins. SHIP1 also contains three SH3-interacting regions (a.a. 124-129, 969-974, 1040-1051) characterized by the presence of the PxxP motifs and two NPXY motifs (a.a. 912-915 & 1019-1022) that interact with SH2 domain-containing proteins.

Especificidad

Immunogen sequence is present in all three spliced isoforms of human SHIP1 reported by UniProt (Q92835).

Inmunógeno

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to a sequence near the C-terminus of human SHIP1.

Aplicación

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected SHIP1 in human lymphoma tissue, as well as in normal tonsil and kidney tissue sections.

Research Category
Neuroscience

Research Sub Category
Kinases & Phosphatases

This Anti-SHIP1 Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of SHIP1.

Calidad

Evaluated by Western Blotting in Ramos cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected SHIP1 in 10 µg of Ramos cell lysate.

Descripción de destino

~145 kDa observed. Target band size appears larger than the calculated molecular weights of 133.63 kDa (isoform 1), 133.2 kDa (isoform 2), 109.3 kDa (isoform 3) due to posttranslational phosphorylation.

Forma física

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1