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ABC464

Sigma-Aldrich

Anti-VAMP-3 Antibody

from rabbit, purified by affinity chromatography

Sinónimos:

Vesicle-associated membrane protein 3, VAMP-3, Cellubrevin, CEB, Synaptobrevin-3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), rat (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... VAMP3(9341)

General description

VAMP-3 also known as cellubrevin is a vesicular transport protein and SNARE protein important in vesicle trafficking and exocytosis that helps regulate the release of critical components and processes such as blood clotting and inflammatory responses through the release of secretory granules and secretory vesicles. VAMP3 regulates the release of WPBs from endothelial cells. Weibel-Palade bodies (WPBs) are secretory organelles of endothelial cells that store the thrombogenic glycoprotein von Willebrand factor (vWF). Endothelial activation, for example by histamine and thrombin, triggers the Ca(2+)-dependent exocytosis of WPB that releases vWF into the vasculature and thereby initiates platelet capture and thrombus (blood clot) formation. VAMP3 is also critical to the release of proinflammatory compounds IL-6 and TNF-alpha.VAMP-3 is widely expressed in cells.

Immunogen

Epitope: Near N-terminus
KLH-conjugated linear peptide corresponding to human VAMP-3 near the N-terminus.

Application

Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-VAMP-3 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of VAMP-3.
Western Blotting Analysis: A 1:500 dilution from a representative lot detected VAMP-3 in 10 µg of Huvec and human brain tissue lysate.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected VAMP-3 in PANC-1 cells.

Quality

Evaluated by Western Blotting in PANC-1 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected VAMP-3 in 10 µg of PANC-1 cell lysate.

Target description

~13 kDa observed.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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