AB9856

Anti-TorsinB Antibody

serum, Chemicon^®

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat, mouse, human
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NM_014506.1
• UniProt accession no.: O14657
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... TOR1B(27348)

Descripción

Specificity

TorsinB. The antibody reacts with the ~38 kDa protein.

Immunogen

Synthetic peptide from torsinB.

Application

Detect TorsinB using this Anti-TorsinB Antibody validated for use in IP, WB & IC.

Western blot: 1:100-1:200. The antibody reacts with the ~38 kDa protein. The suggested blocking buffer is TBS with 10% milk and 0.05% Tween-20. The suggested dilution buffer is TBS with 1% milk and 0.05% Tween-20. Note: TorsinB is a glycoprotein which can be deglycosylated by either EndoH or PNGase treatment.
Immunocytochemistry: 1:100 using rhodamine conjugated goat anti-rabbit secondary antibody. Suggested fixative is 4% paraformaldehyde fixed (37°C for 20 minutes) cells. Suggested blocking solution should contain 10% goat serum. Suggested dilution buffer is PBS containing 1% BSA.
Immunoprecipitation. Recommended antibody amount is 5-7 mL of rabbit serum. The preferred tissue/cell lysis buffer is RIPA. Final reaction volume of 1000 mL. Final total protein concentration in reaction mix: 0.1-0.5 mg/mL. Suggested incubation time is 16-18 hours at 2-8°C. Sugggested capture is Protein A/G.

Optimal working dilutions must be determined by end user.

Storage and Stability

Maintain at frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Western blot: brain and liver. Immunocytochemistry: SH-SY5Y cells.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable