AB5612
Anti-Citrulline Antibody
serum, Chemicon®
Sinónimos:
CCP
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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Technique(s):
ELISA: suitable, immunohistochemistry: suitable
Application:
ELISA, IHC
Citations:
3
Servicio técnico
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Permítanos ayudarlebiological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable
suitability
not suitable for immunohistochemistry (Paraffin)
shipped in
wet ice
target post-translational modification
unmodified
Immunogen
Citrulline-Glutaraldehyde-BSA
Application
Anti-Citrulline Antibody is a Rabbit Polyclonal Antibody for the detection of Citrulline & has been validated in ELISA & IHC.
Immunohistochemistry: 1:1,000-1:2,500 glutaraldehyde required for reactivity. (0.5%-2.5% is typical; fluorescence detection is not recommended. Blocking is with 3% normal serum with 0.1-0.2% triton X-100 usually. Primary antibody is diluted only in TBS or PBS with out blocking or triton X-100. Incubations are usually 3 hours 37C or 4C overnight. Detection is by ABC or PAP for best results. Not recommended for paraffin embedded tissues.
ELISA: 1:2500-1:5000
Optimal working dilutions must be determined by the end user.
ELISA: 1:2500-1:5000
Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Biochem/physiol Actions
Citrulline in glutaraldehyde fixed tissues or cells.
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity ratio
Citrulline-G-BSA 1
Arginine-G-BSA 1/>100,000
Glutamine-G-BSA 1/>100,000
Ornithine-G-BSA 1/>100,000
Abbreviations:
(G) Glutaraldehyde
(BSA) Bovine Serum Albumin
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity ratio
Citrulline-G-BSA 1
Arginine-G-BSA 1/>100,000
Glutamine-G-BSA 1/>100,000
Ornithine-G-BSA 1/>100,000
Abbreviations:
(G) Glutaraldehyde
(BSA) Bovine Serum Albumin
Physical form
Rabbit antiserum. Liquid with 0.05% sodium azide
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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