AB19167

Anti-MMP-2 Antibody, whole molecule

Chemicon^®, from rabbit

• Sinónimos: 72 kDa gelatinase, 72kD type IV collagenase, Gelatinase A, Matrix metalloproteinase-2, collagenase type IV-A, matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), matrix metalloproteinase 2, matrix metalloproteinase 2 (g
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: rabbit
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: bovine, rat
• species reactivity (predicted by homology): human, mouse
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI accession no.: NM_004530.2
• UniProt accession no.: P08253
• shipped in: wet ice
• Gene Information: human ... MMP2(4313)

Descripción

General description

MMPs have a common mode of activation, a conserved amino acid sequence in the putative metal-binding active site region and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). These MMPs and TIMPs could be expressed by either the cancer of the stromal cells. There is a co-operation between tumor and stromal cells, in particular for the production of 72-kD type IV collagenase, involved in the disruption of basement membranes. A lack of TIMP-1 expression from invasive cancer cells could also contribute to matrix destruction.

Specificity

AB19167 recognizes protein at 72 kDa, identified as matrix metalloproteinase 2. MMP-2 is also known as 72 kDa collagenase IV or gelatinase A. It is synthesized as a 631 amino acid proenzyme that is activated by cleavage of the first 80 amino acids. AB19167 shows no cross-reaction with (pro) and active forms of other MMPs. Cytoplasmic localization.

Reactivity with other species has not been confirmed.

Immunogen

A synthetic peptide from the second half of human MMP-2.

Epitope: Whole molecule

Application

Detect MMP-2 using this Anti-MMP-2 Antibody, whole molecule validated for use in IH(P) & WB.

Immunohistochemistry (formalin/paraffin):
10-20 µg/mL of a previous lot was kept for 30 min at RT. No special pretreatment is required for staining formalin-fixed paraffin-embedded sections.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Quality

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected MMP-2 on 10 µg of PC12 lysates.

Target description

72 kDa

Linkage

Replaces: 04-1048

Physical form

Format: Purified

Protein A chromatography

Purified rabbit polyclonal in buffer containing 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
Positive control: Conditioned, serum-free medium from TPA-treated human fetal lung (HFL-1) cells. Placenta or bladder, breast, or ovarian carcinomas.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10-13 - German Storage Class 10 to 13