69820-M

pT7Blue^™ T-Vector - Novagen

designed for simplified cloning of PCR products

• Código UNSPSC: 41106610
• NACRES: NA.51

Propiedades

• Nivel de calidad: 100
• Formulario: liquid
• fabricante / nombre comercial: Novagen^®
• condiciones de almacenamiento: OK to freeze
• temp. de almacenamiento: −20°C

Descripción

Descripción general

The pT7Blue^™ T-Vector is designed for simplified cloning of PCR products. Many thermostable DNA polymerases, such as Taq DNA polymerase, leave single 3′ A-nucleotide overhangs on their reaction products. These molecules can be ligated directly to a vector containing compatible single T-nucleotide overhangs. The pT7Blue^™ vector has been specifically constructed for this application and has been prepared for T-cloning by digestion with EcoRV followed by the addition of single 3′ dT residues at each end. The T-Vector is provided ready to ligate with DNA amplified with any DNA polymerase that leaves single 3′ dA overhangs. Target inserts having other types of ends, such as reaction products of proofreading polymerase or restriction fragments, can be easily prepared for T-cloning with the Single dA<TMSYMBOL></TMSYMBOL> Tailing Kit. pT7Blue^™ contains the pUC19 backbone (including high-copy number origin or replication and lac sequences), a T7 promoter, f1 origin of replication, and a modified multiple cloning region.

Componentes

• 2g pT7Blue^™ T-Vector, 50 ng/l
• 25 ng T-Vector Positive Control Insert, 2.5 ng/l

Advertencia

Toxicity: Standard Handling (A)

Información legal

This product is only available in Japan.

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

PT7BLUE is a trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable