444258

MMP-14 Substrate I, Fluorogenic

A specific fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity.

• Sinónimos: MMP-14 Substrate I, Fluorogenic, MT1-MMP Substrate I, Fluorogenic, MCA-PLA-C(OMeBz)-WAR(Dpa)-NH₂
• Fórmula empírica (notación de Hill): C₆₆H₈₂N₁₆O₁₇S
• Peso molecular: 1403.52
• Código UNSPSC: 12352200
• NACRES: NA.77

Propiedades

• Nivel de calidad: 100
• Ensayo: ≥97% (HPLC)
• Formulario: lyophilized solid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; protect from light
• color: yellow
• solubilidad: DMSO: 5 mg/mL
• fluorescencia: λ_ex ~328 nm; λ_em ~400 nm
• Condiciones de envío: ambient
• temp. de almacenamiento: −20°C

Descripción

Descripción general

A fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity (k_cat/k_m = 730,000 M^-1s^-1 for MMP-14 (MT1-MMP) and 36,000 M^-1s^-1 for MMP-11 (ST3); pH = 7.5 at 25°C). Excitation max.: ~328 nm; emission max.: ~400 nm.

A specific fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity. (k_cat/K_m = 7.3 x 10⁵ M^-1s^-1 for MMP-14 (MT1-MMP) and 3.6 x 10⁴ M^-1s^-1 for MMP-11 at 25°C, pH 7.5).

Acciones bioquímicas o fisiológicas

Cell permeable: no

Primary Target
MMP-14

Product does not compete with ATP.

Reversible: no

kcat/Km = 7.3 x 105 M-1s-1 for MMP-14; .6 x 104 M-1s-1 for MMP-11

Envase

Packaged under inert gas

Advertencia

Toxicity: Standard Handling (A)

Secuencia

MCA-Pro-Leu-Ala-Cys(p-OMeBz)-Trp-Ala-Arg(Dpa)-NH₂ Dpa = N³-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl, Bz = Benzyl

Forma física

Supplied as a trifluoroacetate salt.

Reconstitución

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Otras notas

Mucha, A., et al. 1998. J. Biol. Chem.273, 2763.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable