440319
Lumican, His•Tag®, CF, Mouse, Recombinant
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
UNSPSC Code:
12352203
NACRES:
NA.45
Productos recomendados
assay
≥95% (SDS-PAGE)
Quality Level
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤1.0 EU/μg protein Endotoxin
shipped in
ambient
storage temp.
−20°C
General description
Recombinant, murine lumican, consisting of amino acids 19-338, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells. Lumincan a 40-kDa member of the small leucine-rich repeat proteoglycans (SLRPs) family/Class II subfamily of proteins. It is known for its ability to modulate collagen fibrillogenesis and role in cell proliferation regulation.
Biochem/physiol Actions
Cell adhesion: 50-70% after 50 min at 37°C as measured by the ability of immobilized lumican to supoprt the adhesion of 5 X 10⁴ BCE C/D-1b bovine cornea-derived endothelial cells.
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from 0.2 µm filtered PBS.
Reconstitution
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 200 µg/ml with sterile PBS.
Other Notes
D′Onofrio, M.F. et al. 2008. Biochem. Biophys. Res. Commun.365, 266.
Nikitovic, D. et al. 2008. IUBMB Life60, 818.
Nikitovic, D. et al. 2008. FEBS J.275, 350.
Ishiwata, T. et al. 2007. Oncol. Rep.18, 537.
Vuillermoz, B. et al. 2004. Exp. Cell Res.296,294.
Chakravarti, S. et al. 2003. Invest. Ophthalmol. Vis. Sci.44, 2422.
Jepsen, K.J. et al. 2002. J. Biol. Chem.277, 35532.
Chakravarti, S. et al. 2000. Invest. Ophthalmol. Vis. Sci.41,3365.
Blochberger, T.C. et al. 1992. J. Biol. Chem.267, 347.
Chakravarti, S. et al. 1998. J. Cell Biol.141, 1277.
Nikitovic, D. et al. 2008. IUBMB Life60, 818.
Nikitovic, D. et al. 2008. FEBS J.275, 350.
Ishiwata, T. et al. 2007. Oncol. Rep.18, 537.
Vuillermoz, B. et al. 2004. Exp. Cell Res.296,294.
Chakravarti, S. et al. 2003. Invest. Ophthalmol. Vis. Sci.44, 2422.
Jepsen, K.J. et al. 2002. J. Biol. Chem.277, 35532.
Chakravarti, S. et al. 2000. Invest. Ophthalmol. Vis. Sci.41,3365.
Blochberger, T.C. et al. 1992. J. Biol. Chem.267, 347.
Chakravarti, S. et al. 1998. J. Cell Biol.141, 1277.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico