420411

Kanamycin Sulfate, Streptomyces kanamyceticus, Cell Culture-Tested

An aminoglycoside antibiotic effective against Gram-positive and Gram-negative organisms. Inhibitor of protein biosynthesis that acts on the 30S ribosome, causing misreading of the genetic code.

• Sinónimos: Kanamycin Sulfate, Streptomyces kanamyceticus, Cell Culture-Tested
• Fórmula empírica (notación de Hill): C₁₈H₃₆N₄O₁₁ · xH₂SO₄
• Número de CAS: 25389-94-0
• Peso molecular: 484.50 (free base basis)
• Código UNSPSC: 12352200

Propiedades

• Nivel de calidad: 100
• Formulario: solid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• color: white to off-white
• solubilidad: water: 10 mg/mL
• Condiciones de envío: ambient
• temp. de almacenamiento: 10-30°C
• InChI: 1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1
• Clave InChI: OOYGSFOGFJDDHP-KMCOLRRFSA-N

Descripción

Descripción general

Recommended working concentration: 100 µg/ml.

An aminoglycoside antibiotic effective against Gram-positive and Gram-negative organisms. Inhibitor of protein biosynthesis that acts on the 70S ribosome, causing misreading of the genetic code. May cause renal damage and ototoxicity. Useful for the selection of kanamycin resistant plasmids in E. coli. Tested and found to be negative for cytotoxicity against HeLa cells. Contains more than 98% kanamycin A.

Contains >98% kanamycin A. Potency: ≥750 µg/mg

Acciones bioquímicas o fisiológicas

Primary Target
protein biosynthesis that acts on the 30S ribosome

Advertencia

Toxicity: Carcinogenic / Teratogenic (D)

Reconstitución

Following reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.

Otras notas

Lech, K., and Brent, R. 1995. in Current Protocols in Mol. Biol. Vol. 1 (Ausubel, F.M., et al., Eds.) John Wiley & Sons, New York.
Cardon, G.H., et al. 1993. Plant J.3, 773.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York. p.1.6, A.6.
Korzybyski, T., et al. 1978. In Antibiotics, Origin, Nature, and Properties1, 690.
Stiffler, P.W., et al. 1974. Antimicrob. Agents Chemother.6, 516.
Umezawa, S., et al. 1968. J. Antibiot.21, 367.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H360D
• Consejos de prudencia: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Clasificaciones de peligro: Repr. 1B
• Código de clase de almacenamiento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Clase de riesgo para el agua (WGK): WGK 2