375228

Hepatocyte Growth Factor, Human, Recombinant, S. frugiperda

• Sinónimos: rhHGF
• Código UNSPSC: 12352202

Propiedades

• Ensayo: ≥95% (SDS-PAGE)
• Nivel de calidad: 100
• Formulario: lyophilized
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• impurezas: ≤1 EU/μg endotoxin (EU/μg HGF)
• Condiciones de envío: ambient
• temp. de almacenamiento: −70°C

Descripción

Descripción general

Recombinant, human hepatocyte growth factor expressed in S. frugiperda insect cells. Pleiotropic growth factor that has also been called scatter factor, hepatopoietin A, and mammary growth factor. Through binding to its high affinity met receptor, HGF has marked and varied effects on hepatocytes and other epithelial and endothelial cell types. HGF-induced proliferation of primary hepatocytes is mediated by activation of PI 3-kinase. Acts as a potent mitogen for mature hepatocytes.

Recombinant, human hepatocyte growth factor expressed in Spodoptera frugiperda insect cells. Pleiotropic growth factor that has also been called scatter factor, hepatopoietin A, and mammary growth factor. Through binding to its high affinity met receptor, HGF has marked and varied effects on hepatocytes and other epithelial and endothelial cell types. HGF-induced proliferation of primary hepatocytes is mediated by activation of PI 3-kinase. Acts as a potent mitogen for mature hepatocytes.

Acciones bioquímicas o fisiológicas

ED₅₀ = 20-40 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation in an HGF-responsive epithelial cell line, 4MBr-5

Advertencia

Toxicity: Standard Handling (A)

Forma física

Lyophilized from a sterile-filtered solution in 20 mM Sodium Phosphate, 0.5 M NaCl, 50 µg BSA/µg HGF, pH 7.0.

Reconstitución

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥1 µg/ml in sterile PBS containing ≥0.1% HSA or BSA.

Otras notas

Kaido, T., et al. 1996. Biochem. Biophys. Res. Commun. 218, 1.
Kobayashi, Y., et al. 1996. Biochem. Biophys. Res. Commun. 220, 7.
Skouteris, G.G., et al. 1996. Biochem. Biophys. Res. Commun. 218, 229.
Rubin, J.S., et al. 1993. Biochim. Biophys. Acta 1155, 357.
Strain, A.J. 1993. J. Endocrinol. 137, 1. Nakamura, T., et al. 1989. Nature 342, 440.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3