341595
FGF-2, Basic, Human, Recombinant, E. coli
Sinónimos:
FGF-2, Basic, Human, Recombinant, E. coli, rhbFGF, Fibroblast Growth Factor, Basic, Human, Recombinant, E. coli
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About This Item
Ensayo
≥97% (SDS-PAGE)
Nivel de calidad
Formulario
lyophilized
fabricante / nombre comercial
Calbiochem®
condiciones de almacenamiento
OK to freeze
impurezas
≤1 EU/μg Endotoxin (EU/μg FGF)
Condiciones de envío
ambient
temp. de almacenamiento
−70°C
Descripción general
Recombinant, human basic fibroblast growth factor expressed in E. coli. A member of the FGF family of mitogenic peptides, comprised of seven related proteins showing 35-55% amino acid conservation within the family and 55% similarity with acidic FGF. Found in neuronal tissue (hippocampus, brain stem, peripheral ganglia, and the isocortex), pituitary, adrenal cortex, corpus luteum, and placenta. Some of the biological activities attributed to basic FGF include: mitogenic activity in vitro for cells of meso- and neuroectodermal origin (i.e. fibroblasts, endothelial cells, astrocytes, oligodendrocytes, neuroblasts, keratinocytes, bovine epithelial lens cells, osteoblasts, smooth muscle cells, and melanocytes); role in wound healing; induction of neuron differentiation, survival, and regeneration; chemoattractant effect and mitogenic activity in vitro for endothelial cells and angiogenic activity in vivo; role in tumor neovascularization. Inhibits DNA synthesis and alkaline phosphatase activity in rat osteosarcoma cell lines. Has been implicated in the development of Kaposi sarcoma in vitro. Inhibits iNOS in bovine retinal pigmented epithelial cells. Binding of FGF to heparin or cell surface heparin sulfate proteoglycans is necessary for FGF binding to its high affinity receptors. Basic and acidic FGF appear to bind to the same high affinity receptors and show a similar range of biological activities.
Recombinant, human basic fibroblast growth factor expressed in E. coli. Potent mitogen for bone cells. Inhibits DNA synthesis and alkaline phosphatase activity in rat osteosarcoma cell lines. Has been implicated in the development of Kaposi sarcoma in vitro. Inhibits inducible NOS in bovine retinal pigmented epithelial cells.
Acciones bioquímicas o fisiológicas
ED₅₀ = 100-250 pg/ml as monitored in a mitogenic assay by measuring FGF basic-dependent ³H-thymidine incorporation in quiescent NR6R/3T3 fibroblasts
Advertencia
Toxicity: Standard Handling (A)
Forma física
Lyophilized from sterile-filtered 20 mM Tris-HCl, 1 M NaCl, 50 µg BSA/1 µg cytokine, pH 7.0.
Reconstitución
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 6 months at -70°C.
Reconstitute to a concentration ≥10 µg/ml with sterile PBS containing 1 mM DTT and ≥0.1% HSA or BSA.
Otras notas
Goureau, O., et al. 1995. Eur. J. Biochem. 230, 1046.
Gibran, N.S., et al. 1994. J. Surg. Res. 56, 226.
Li, J.J., et al. 1993. Cancer72, 2253.
Mignatti, P., et al. 1992. J. Cell Physiol.151, 81.
Mignatti, P., et al. 1991. Proc. Natl. Acad. Sci. USA88, 11007.
Baird, A., and Klagsbrun, M. 1991. Cancer Cells3, 239.
Gospodarowicz, D. 1991. Ann. NY Acad. Sci.638, 1.
Basilico, C., et al. 1989. Ann. NY Acad. Sci.567, 95.
Burgess, W.H., and Maciag, T. 1989. Annu. Rev. Biochem.58, 575.
Rogelj, S., et al. 1989. J. Cell. Biochem.39, 13.
Rizzino, A., et al. 1988. Cancer Res.48, 4266.
Abraham, J.A., et al. 1986. EMBO J.5, 2523.
Gibran, N.S., et al. 1994. J. Surg. Res. 56, 226.
Li, J.J., et al. 1993. Cancer72, 2253.
Mignatti, P., et al. 1992. J. Cell Physiol.151, 81.
Mignatti, P., et al. 1991. Proc. Natl. Acad. Sci. USA88, 11007.
Baird, A., and Klagsbrun, M. 1991. Cancer Cells3, 239.
Gospodarowicz, D. 1991. Ann. NY Acad. Sci.638, 1.
Basilico, C., et al. 1989. Ann. NY Acad. Sci.567, 95.
Burgess, W.H., and Maciag, T. 1989. Annu. Rev. Biochem.58, 575.
Rogelj, S., et al. 1989. J. Cell. Biochem.39, 13.
Rizzino, A., et al. 1988. Cancer Res.48, 4266.
Abraham, J.A., et al. 1986. EMBO J.5, 2523.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
P Mignatti et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(24), 11007-11011 (1991-12-15)
Basic fibroblast growth factor (bFGF), a protein with angiogenic, mitogenic, and chemotactic properties, lacks a signal sequence and is not secreted via the classical secretory pathway. However, the growth factor is known to act extracellularly. Since no defined mechanism for
Biological activities of fibroblast growth factors.
D Gospodarowicz
Annals of the New York Academy of Sciences, 638, 1-8 (1991-01-01)
S Rogelj et al.
Journal of cellular biochemistry, 39(1), 13-23 (1989-01-01)
Basic fibroblast growth factor (bFGF) is found in a variety of cells and tissues. We have previously shown that bFGF is a transforming growth factor, but only when fused to a signal peptide (sp-bFGF). Cells expressing the native bFGF are
Expression and activation of the K-fgf oncogene.
C Basilico et al.
Annals of the New York Academy of Sciences, 567, 95-103 (1989-01-01)
The fibroblast growth factor family.
A Baird et al.
Cancer cells (Cold Spring Harbor, N.Y. : 1989), 3(6), 239-243 (1991-06-01)
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