234401
Complement C1q-Depleted Serum, Human
Sinónimos:
C1q-Depleted Serum, Human Complement Serum
About This Item
Productos recomendados
form
solution
Quality Level
does not contain
preservative
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
impurities
C1q, none detected (Ouchterlony using various dilutions of goat anti-human C1q antiserum.)
color
clear amber
shipped in
dry ice
storage temp.
−70°C
General description
Packaging
Warning
Unit Definition
Physical form
Preparation Note
Reconstitution
Other Notes
CLASSICAL PATHWAY ACTIVITY: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 107 EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 minutes at 37°C in a total reaction volume of 500 μl GVB++.
• Recommended volume of C1q-Dpl serum per assay
• C1qH50 units/mg purified C1q at the recommended input of C1q-Dpl
• Input of purified C1q to yield 1 C1qH50:
• C1qH50 units/ml NHS Complement standard at the recommended input of C1q-Dpl
• Input of C1q in NHS Complement standard to yield 1 C1qH50
• Ratio
• Background A412 EA blank reading at the recommended input of C1q-Dpl
CH50 FUNCTIONAL ACTIVITY UPON RECONSTITUTION: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 108 EA when incubated for 60 minutes at 37°C in a total reaction volume of 1.5 ml GVB++.
• CH50/ml NHS Standard
• CH50/ml C1q-Dpl reconstituted with 100 μg C1q/ml:
• Ratio
ALTERNATIVE PATHWAY ACTIVITY: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 107 rabbit erythrocytes when incubated for 30 minutes at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.
• APH50/ml NHS Standard
• Input of NHS Standard to yield 1 APH50
• APH50/ml C1q-Dpl
• Input of C1q-Dpl to yield 1 APH50
• AP Ratio
Legal Information
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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