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12-342

Rabbit Anti-Sheep IgG Antibody, HRP conjugate

Upstate®, from rabbit

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Talla/SKUDisponibilidadPrecio
500 μg
Comprobar disponibilidad del carrito
$264.00

Acerca de este artículo

UNSPSC Code:
12352203
NACRES:
NA.46
eCl@ss:
32160702
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA, IHC, WB
Citations:
29

$264.00


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biological source

rabbit

Quality Segment

conjugate

peroxidase conjugate

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

sheep

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Application

Rabbit anti-Sheep IgG Antibody, HRP conjugate detects level of Sheep IgG & has been published & validated for use in ELISA, WB, IH.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Physical form

0.02M Potassium Phosphate, 0.15M NaCl, pH 7.2, 10mg/ml BSA, and 0.01% gentamicin sulfate

Preparation Note

Lyophilized: 2 years at 4°C; Rehydrated: 6 months at -20°C.

Analysis Note

Routinely evaluated by western blot

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Questions

1–2 of 2 Questions  
  1. Could you please provide the reconstitution method for this product and the recommended concentration for various uses?

    1 answer
    1. To prepare this product, add 500µl of ultrapure water to make a 1mg/ml stock solution. It is recommended to aliquot the solution to avoid repeated freezing and thawing. The reconstituted solution can be stored for up to 6 months at -20°C. This product is suitable for use in immunohistochemistry at a 1:1000-1:4000 dilution. You can review available Certificates of Analysis by visiting the documentation section on our website.

      Helpful?

  2. What is the method of reconstitution for this product and what is the concentration recommended for various uses?

    1 answer
    1. This product can be prepared by adding 500µl of ultrapure water to make a 1mg/ml stock solution. Aliquot to avoid repeated freezing and thawing. The reconstituted solution may be stored up to 6 months at -20°C. This product is tested for use in immunohistochemistry, at which a 1:1000-1:4000 dilution is suitable. See the link below to review available Certificates of Analysis: https://www.emdmillipore.com/product/Rabbit-Anti-Sheep-IgG-Antibody-HRP-conjugate,MM_NF-12-342#documentation

      Helpful?

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