116818

Adenosine 3′,5′-cyclic Monophosphorothioate, 8-Bromo-, Sp-Isomer, Sodium Salt

A potent cell-permeable, metabolically-stable activator of cAMP-dependent protein kinase (PKA).

• Sinónimos: Adenosine 3′,5′-cyclic Monophosphorothioate, 8-Bromo-, Sp-Isomer, Sodium Salt, Sp-8-Br-cAMPS, Na
• Fórmula empírica (notación de Hill): C₁₀H₁₀BrN₅O₅PS · Na
• Peso molecular: 446.15
• UNSPSC Code: 12352200

Propiedades

• Quality Level: 100
• assay: ≥99% (HPLC)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• solubility: water: 40 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Descripción

General description

A potent cell-permeable, metabolically stable activator of cAMP-dependent protein kinase (PKA). Induces the guanine-nucleotide-exchange factor activity of GEFI, also known as Epac (exchange protein directly activated by cAMP), and GEFII towards Rap1 both in vivo and in vitro. Significantly more lipophilic and cell permeable than Sp-cAMPS (Cat. No. 116815). λ_max = 264 nm (H₂O, pH 7.0); ε_max = 17,000 M^-1cm^-1. Note: 5 µmol = 2.23 mg.

A potent cell-permeable, metabolically-stable activator of cAMP-dependent protein kinase (PKA). Induces guanine nucleotide exchange factor activity of GEFI (also known as Epac) and GEFII towards Rap1 both in vivo and in vitro. Significantly more lipophilic and cell-permeable than Sp-cAMPS (Cat. No. 116815). Note: 5 µmol = 2.23 mg.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
PKA

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Kawasaki, H., et al. 1998. Science 282, 2275.
de Rooij, J., et al. 1998. Nature 396, 474.
Holen, I., et al. 1996. Eur. J. Biochem. 236, 163.
Schaap, P., et al. 1993. J. Biol. Chem.268, 6323.
Yokozaki, H., et al. 1992. Cancer Res.52, 2504.

Legal Information

Sold under license of Patent DE 3,802,865.4 issued to BIOLOG LSI.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable