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09-872

Sigma-Aldrich

Anti-Methylcytosine dioxygenase TET1 Antibody

from rabbit, purified by affinity chromatography

Sinónimos:

tet oncogene 1, Ten-eleven translocation 1 gene protein, CXXC-type zinc finger protein 6, methylcytosine dioxygenase TET1, CXXC finger 6, ten-eleven translocation-1, Leukemia-associated protein with a CXXC domain

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

tipo de anticuerpo

primary antibodies

clon

polyclonal

purificado por

affinity chromatography

reactividad de especies

mouse

técnicas

ChIP: suitable (ChIP-seq)
western blot: suitable

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

Información sobre el gen

human ... TET1(80312)

Descripción general

Methylcytosine dioxygenase TET1 (mTET1) is a member of the TET family and contains a characteristic CXXC-type zinc finger domain. mTET1 binds methylcytosine (5mC), a minor, mammalian DNA base necessary to development and retrotransposon silencing, and acts as a catalyst to induce the conversion of 5mC to 5-hydromethylcytosine (hmC or 5hmC), a mammalian DNA base thought to be involved in chromatin structure and in the development of fetal brain, heart and lung tissues. Recent studies indicate that mTET1 regulates the levels of 5hmC, thereby influencing patterns in DNA methylation.

Especificidad

Other homologies: Rat (66% sequence homology), Human (61% sequence homology).
This antibody recognizes methylcytosine dioxygenase TET1 near the C-terminus.

Inmunógeno

Epitope: Near the C-terminus
KLH-conjugated linear peptide corresponding to mouse Methylcytosine dioxygenase TET1 near the C-terminus.

Aplicación

Anti-Methylcytosine dioxygenase TET1 Antibody is a Rabbit Polyclonal Antibody for detection of Methylcytosine dioxygenase TET1 also known as tet oncogene 1 & has been validated in WB, ChIP-seq.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histone Modifying Proteins
Western Blot (Snap i.d.) Analysis: 5 µg/mL from a previous lot detected Methylcytosine dioxygenase TET1 in 10 µg of mouse embryonic stem cell lysate.

Calidad

Evaluated by Western Blot in mouse embryonic stem cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Methylcytosine dioxygenase TET1 in 10 µg of mouse embryonic stem cell lysate.

Descripción de destino

~220 kDa observed.
Uncharacterized bands may be seen at ~32, 68, and 70 kDa

Forma física

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Mouse embryonic stem cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Pietro Vella et al.
Molecular cell, 49(4), 645-656 (2013-01-29)
O-linked N-acetylglucosamine (O-GlcNAc) transferase (Ogt) activity is essential for embryonic stem cell (ESC) viability and mouse development. Ogt is present both in the cytoplasm and the nucleus of different cell types and catalyzes serine and threonine glycosylation. We have characterized
Suppression of TET1-dependent DNA demethylation is essential for KRAS-mediated transformation.
Wu, BK; Brenner, C
Cell Reports null
Dynamic hydroxymethylation of deoxyribonucleic acid marks differentiation-associated enhancers.
Serandour, Aurelien A, et al.
Nucleic Acids Research (2012)
Alain R Weber et al.
Nature communications, 7, 10806-10806 (2016-03-05)
Cytosine methylation in CpG dinucleotides is an epigenetic DNA modification dynamically established and maintained by DNA methyltransferases and demethylases. Molecular mechanisms of active DNA demethylation began to surface only recently with the discovery of the 5-methylcytosine (5mC)-directed hydroxylase and base
Yabin Wei et al.
The international journal of neuropsychopharmacology, 18(2) (2015-01-27)
Epigenetic drugs like sodium butyrate (NaB) show antidepressant-like effects in preclinical studies, but the exact molecular mechanisms of the antidepressant effects remain unknown. While research using NaB has mainly focused on its role as a histone deacetylase inhibitor (HDACi), there

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