05-1570

Anti-PRDM1 (BLIMP-1) Antibody, clone 6D3

clone 6D3, from rat

• Sinónimos: PR domain containing 1, with ZNF domain, PR-domain zinc finger protein 1, beta-interferon gene positive-regulatory domain I binding factor, Positive regulatory domain I-binding factor 1, PRDI-binding factor-1, Beta-interferon gene positive regulatory dom
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rat
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 6D3, monoclonal
• reactividad de especies: mouse, human
• técnicas: ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG2aκ
• Nº de acceso NCBI: NP_031574
• Nº de acceso UniProt: Q60636
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PRDM1(639)

Descripción

Descripción general

B-lymphocyte induced maturation protein (BLIMP-1) is a 100 kDa transcriptional repressor containing 1 DET domain and 4 C2H2-type zinc fingers. In the nucleus, BLIMP-1 is known to bind specifically with the Beta-interferon gene positive regulatory domain I (PRDI), and to interact with PRMT5. During B-cell differentiation, BLIMP-1 actively represses the transcription of the gene, c-myc, through binding its promoter and has been deemed a “master regulator” for terminal B-cell differentiation, as it is able to initiate the BCL1 cell developmental cascade.

Especificidad

Recognizes BLIMP-1

Inmunógeno

Epitope: Recognizes epitope located between amino acids 255-395 of BLIMP-1

Recombinant protein corresponding to mouse BLIMP-1.

Aplicación

ELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (Kallies, A., et al. (2004). J Exp Med. 200(8):967-977.)

Flow Cytometry Analysis: A representative lot was used by an independent laboratory in FC. (Kallies, A., et al. (2004). J Exp Med. 200(8):967-977.)

Research Category
Epigenetics & Nuclear Function

Inflammation & Immunology

Use Anti-PRDM1 (BLIMP-1) Antibody, clone 6D3 (Rat Monoclonal Antibody) validated in WB, ELISA, IHC, FC to detect PRDM1 (BLIMP-1) also known as PR-domain zinc finger protein 1.

Calidad

Evaluated by Western Blot in HL-60 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected BLIMP-1 on 10 µg of HL-60 cell lysate.

Descripción de destino

~ 95 kDa observed

Forma física

Format: Purified

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable