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05-1352

Sigma-Aldrich

Anti-Histone H2B Antibody, clone 5HH2-2A8

ascites fluid, clone 5HH2-2A8, from mouse

Sinónimos:

H2B, Histone H2B, histone 2, H2be, histone cluster 2, H2be, H2B histone family, member Q

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100 μL
$616.00

$616.00


Fecha estimada de envío12 de mayo de 2025


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100 μL
$616.00

About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

$616.00


Fecha estimada de envío12 de mayo de 2025


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origen biológico

mouse

Nivel de calidad

forma del anticuerpo

ascites fluid

tipo de anticuerpo

primary antibodies

clon

5HH2-2A8, monoclonal

reactividad de especies

mouse, human, rat

técnicas

immunocytochemistry: suitable
western blot: suitable

isotipo

IgG1κ

Nº de acceso GenBank

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... H2BC21(8349)
mouse ... H2Bc21(319190)

Descripción general

Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Especificidad

Antibody recognizes Histone H2B near the C-terminus.

Inmunógeno

Epitope: C-terminus
Ovalbumin-conjugated synthetic linear peptide corresponding to the C-terminus of Histone H2B.

Aplicación

Anti-Histone H2B Antibody, clone 5HH2-2A8 is a Mouse Monoclonal Antibody for detection of Histone H2B also known as Histone H2B, histone 2 H2be & has been validated in WB, ICC.
Immunocytochemistry:
A 1:500 dilution of a representative lot of this antibody detected Histone H2B in A431, HeLa and NIH/3T3 cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Calidad

Evaluated by western blot in HepG2 cell lysate.

Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected Histone H2B in 10 µg of HepG2 cell lysate.

Descripción de destino

~15 kDa

Ligadura / enlace

Replaces: MABE15

Forma física

Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
Unpurified

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análisis

Control
HepG2 cell lysate, L6 cell lysate, Mouse Brain Lysate

Información legal

GenBank is a registered trademark of United States Department of Health and Human Services

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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