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330002S

Avanti

E06 mAb-biotinylated

E06 Mouse Monoclonal Antibody (IgM), Biotinylated Anti-(Oxidized Phospholipid), 1X TBS (Tris Buffered Saline) with 1%BSA

Sinónimos:

E06 mAb-biotinylated, T15 antibody

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.45

forma del anticuerpo

purified from hybridoma cell culture

tipo de anticuerpo

primary antibodies

Ensayo

>95%

Formulario

solution

envase

pkg of 1 × 0.125 mL (polypropylene tube with screw cap (330002S-25ug))

fabricante / nombre comercial

Avanti Research™ - A Croda Brand 330002S

concentración

0.2 mg/mL (330002S-25ug)

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Descripción general

Avanti now provides high purity E06 from an animal-free culture of hybridoma cells. The E06 antibody was developed by Dr. Joseph Witztum at the University of California, San Diego using apolipoprotein E knock-out mice fed a high-fat diet for seven months.

Aplicación

E06 mAb-biotinylated has been used in immunodetection.[1] It may be used in immunohistochemistry,[2] western blot analysis and in in vivo imaging with magnetic resonance imaging (MRI) techniques.
The E06-Biotin is primarily used for sandwich ELISA determination of ox-LDL in serum or plasma samples. It can also be used in direct ELISA, competitive (IHC) and Western Blot analysis. For use in direct ELISA, make a 4 μg/mL working solution of 12 μg E06-Biotin in 3 mL of 1% BSA in PBS (1:62.5 dilution). Make a serial (1:1) dilution of this working solution to titrate the antibody. For competitive ELISA make a 2.5 μg/mL working solution (1:100 dilution).

Acciones bioquímicas o fisiológicas

The E06 antibody is a murine monoclonal IgM natural antibody that binds to oxidized phospholipid and reacts with oxidized Low-density lipoprotein (LDL),[3] oxidized High-density lipoprotein (HDL) and protein covalently modified by oxidized phospholipid. This antibody has been used to probe atherosclerotic plaque in rabbits, monkeys,[2] mice, zebrafish and humans. It also binds to apoptotic cells, but not viable cells.

Envase

0.5 mL Polypropylene Tube with Screw Cap (330002S-25ug)

Información legal

Avanti Research is a trademark of Avanti Polar Lipids, LLC

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Cloning of monoclonal autoantibodies to epitopes of oxidized lipoproteins from apolipoprotein E-deficient mice. Demonstration of epitopes of oxidized low density lipoprotein in human plasma.
Palinski W, et al.
The Journal of Clinical Investigation, 98(3), 800-814 (1996)
Increased plasma oxidized phospholipid: apolipoprotein B-100 ratio with concomitant depletion of oxidized phospholipids from atherosclerotic lesions after dietary lipid-lowering: a potential biomarker of early atherosclerosis regression
Tsimikas S, et al.
Arteriosclerosis, Thrombosis, and Vascular Biology, 27(1), 175-181 (2007)
Impact of inhibition of the autophagy-lysosomal pathway on biomolecules carbonylation and proteome regulation in rat cardiac cells
Coliva G, et al.
Redox Biology, 23, 101123-101123 (2019)

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