Home Mammalian Cell CultureDulbecco's Modified Eagle's Medium (DMEM) Formulation

Dulbecco's Modified Eagle's Medium (DMEM) Formulation

Many composition modifications of Eagle's Medium have been developed since the original formulation appeared in the literature. Among the most widely used of these modifications is Dulbecco's Modified Eagle's medium (DMEM). DMEM is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DMEM formula composition contains 1000 mg/L of glucose and was first reported for culturing embryonic mouse cells. A further alteration with 4500 mg/L glucose has proved to be optimal for the cultivation of certain cell types.

View all DMEM products and filter by glucose, L-glutamine, and other components.

Component	D0422 [1x] g/L	D1145 [1x] g/L
Inorganic Salts
Calcium Chloride	0.2	0.2
Ferric Nitrate • 9H₂O	0.0001	0.0001
Magnesium Sulfate (anhydrous)	0.09767	0.09767
Potassium Chloride	0.4	0.4
Sodium Bicarbonate	3.7	3.7
Sodium Chloride	6.4	6.4
Sodium Phosphate Monobasic (anhydrous)	0.109	0.109
Amino Acids
L-Arginine • HCl	0.084	0.084
L-Cystine • 2HCl	—	0.0626
Glycine	0.03	0.03
L-Histidine • HCl • H₂O	0.042	0.042
L-Isoleucine	0.105	0.105
L-Leucine	0.105	0.105
L-Lysine • HCl	1.46	0.146
L-Methionine	—	0.03
L-Phenylalanine	0.066	0.066
L-Serine	0.042	0.042
L-Threonine	0.095	0.095
L-Tryptophan	0.016	0.016
L-Tyrosine • 2Na •2H₂O	0.12037	0.12037
L-Valine	0.094	0.094
Vitamins
Choline Chloride	0.004	0.004
Folic Acid	0.004	0.004
myo-Inositol	0.0072	0.0072
Niacinamide	0.004	0.004
D-Pantothenic Acid (hemicalcium)	0.004	0.004
Pyridoxal • HCl	—	—
Pyridoxine • HCl	0.004	0.004
Riboflavin	0.0004	0.0004
Thiamine • HCl	0.004	0.004
Other
D-Glucose	4.5	4.5
Phenol Red • Na	0.0159	—
Pyruvic Acid • Na	0.11	—
Add
L-Glutamine	0.584	0.584

Component	D0819 [1x] g/L	D1152 [1x] g/L	D2429 [10x] g/L	D2902 g/L	D5030 g/L	D5523 g/L
Inorganic Salts
Calcium Chloride	0.2	0.2	2	0.2	0.2	0.2
Ferric Nitrate • 9H₂O	0.0001	0.0001	0.001	0.0001	0.0001	0.0001
Magnesium Sulfate (anhydrous)	0.09767	0.09767	0.9767	0.09767	0.09767	0.09767
Potassium Chloride	0.4	0.4	4	0.4	0.4	0.4
Sodium Bicarbonate	3.7	—	—	—	—	—
Sodium Chloride	6.4	4.4	64	6.4	6.4	6.4
Sodium Phosphate Monobasic (anhydrous)	0.109	0.109	1.09	0.109	0.109	0.109
Amino Acids
L-Alanyl-L-Glutamine	0.869	—	—	—	—	—
L-Arginine • HCl	0.084	0.084	0.84	0.084	0.084	0.084
L-Cystine • 2HCl	0.0626	0.0626	0.626	0.0626	0.0626	0.0626
L-Glutamine	—	0.584	—	0.584	—	0.584
Glycine	0.03	0.03	0.3	0.03	0.03	0.03
L-Histidine • HCl • H₂O	0.042	0.042	0.42	0.042	0.042	0.042
L-Isoleucine	0.105	0.105	1.05	0.105	0.105	0.105
L-Leucine	0.105	0.105	1.05	0.105	0.105	0.105
L-Lysine • HCl	0.146	0.146	1.46	0.146	0.146	0.146
L-Methionine	0.03	0.03	0.3	0.03	0.03	0.03
L-Phenylalanine	0.066	0.066	0.66	0.066	0.066	0.066
L-Serine	0.042	0.042	0.42	0.042	0.042	0.042
L-Threonine	0.095	0.095	0.95	0.095	0.095	0.095
L-Tryptophan	0.016	0.016	0.16	0.016	0.016	0.016
L-Tyrosine • 2Na • 2H₂O	0.10379	0.10379	1.0379	0.10379	0.10379	0.10379
L-Valine	0.094	0.094	0.94	0.094	0.094	0.094
Vitamins
Choline Chloride	0.004	0.004	0.04	0.004	0.004	0.004
Folic Acid	0.004	0.004	—	0.004	0.004	0.004
myo-Inositol	0.072	0.0072	0.072	0.0072	0.0072	0.0072
Niacinamide	0.004	0.004	0.04	0.004	0.004	0.004
D-Pantothenic Acid (hemicalcium)	0.004	0.004	0.04	0.004	0.004	0.004
Pyridoxal • HCl	—	0.004	—	0.004	0.004	0.004
Pyridoxine • HCl	0.004	—	0.04	—	—	—
Riboflavin	0.0004	0.0004	0.004	0.0004	0.0004	0.0004
Thiamine • HCl	0.004	0.004	0.04	0.004	0.004	0.004
Other
D-Glucose	4.5	4.5	10	1	—	1
HEPES	—	5.958	—	—	—	—
Phenol Red • Na	0.0159	0.0159	0.159	—	—	0.0159
Pyruvic Acid • Na	—	—	1.1	0.11	—	0.11
Add
Glucose	—	—	—	—	1	—
L-Glutamine	—	—	0.584 at 1×	—	0.584	—
Sodium Bicarbonate	—	3.7	3.7 at 1×	3.7	3.7	3.7

Component	D5546 [1x] g/L	D5648 g/L	D5671 [1x] g/L	D5796 [1x] g/L	D5921 [1x] g/L
Inorganic Salts
Calcium Chloride	0.2	0.2	0.2	0.2	0.2
Ferric Nitrate • 9H₂O	0.0001	0.0001	0.0001	0.0001	0.0001
Magnesium Sulfate (anhydrous)	0.09767	0.09767	0.09767	0.09767	0.09767
Potassium Chloride	0.4	0.4	0.4	0.4	0.4
Sodium Bicarbonate	3.7	—	3.7	3.7	3.7
Sodium Chloride	6.4	6.4	6.4	6.4	6.4
Sodium Phosphate Monobasic (anhydrous)	0.109	0.109	0.109	0.109	0.109
Amino Acids
L-Arginine • HCl	0.084	0.084	0.084	0.084	0.084
L-Cystine • 2HCl	0.0626	0.0626	0.0626	0.0626	0.0626
L-Glutamine	—	0.584	—	0.584	—
Glycine	0.03	0.03	0.03	0.03	0.03
L-Histidine • HCl • H₂O	0.042	0.042	0.042	0.042	0.042
L-Isoleucine	0.105	0.105	0.105	0.105	0.105
L-Leucine	0.105	0.105	0.105	0.105	0.105
L-Lysine • HCl	0.146	0.146	0.146	0.146	0.146
L-Methionine	0.03	0.03	0.03	0.03	0.03
L-Phenylalanine	0.066	0.066	0.066	0.066	0.066
L-Serine	0.042	0.042	0.042	0.042	0.042
L-Threonine	0.095	0.095	0.095	0.095	0.095
L-Tryptophan	0.016	0.016	0.016	0.016	0.016
L-Tyrosine • 2Na • 2H₂O	0.10379	0.10379	0.10379	0.10379	0.12037
L-Valine	0.094	0.094	0.094	0.094	0.094
Vitamins
Choline Chloride	0.004	0.004	0.004	0.004	0.004
Folic Acid	0.004	0.004	0.004	0.004	0.004
myo-Inositol	0.0072	0.0072	0.0072	0.0072	0.0072
Niacinamide	0.004	0.004	0.004	0.004	0.004
D-Pantothenic Acid (hemicalcium)	0.004	0.004	0.004	0.004	0.004
Pyridoxal • HCl	—	0.004	—	—	—
Pyridoxine • HCl	0.004	—	0.004	0.004	0.004
Riboflavin	0.0004	0.0004	0.0004	0.0004	0.0004
Thiamine • HCl	0.004	0.004	0.004	0.004	0.004
Other
D-Glucose	1	4.5	4.5	4.5	1
HEPES	—	—	—	—	—
Phenol Red • Na	0.0159	0.0159	0.0159	0.0159	—
Pyruvic Acid • Na	0.11	—	—	—	—
Add
Glucose	—	—	—	—	—
L-Glutamine	0.584	—	0.584	—	0.584
Sodium Bicarbonate	—	3.7	—	—	—

Component	D6046 [1x] g/L	D6171 [1x] g/L	D6429 [1x] g/L	D6546 [1x] g/L	D7777 g/L	D9443 [1x] g/L
Inorganic Salts
Calcium Chloride	0.2	0.2	0.2	0.2	0.2	0.2
Ferric Nitrate • 9H₂O	0.0001	0.0001	0.0001	0.0001	0.0001	0.0001
Magnesium Sulfate (anhydrous)	0.09767	0.09767	0.09767	0.09767	0.09767	0.09767
Potassium Chloride	0.4	0.4	0.4	0.4	0.4	0.4
Sodium Bicarbonate	3.7	3.7	3.7	3.7	—	3.7
Sodium Chloride	6.4	4.4	6.4	6.4	6.4	6.4
Sodium Phosphate Monobasic (anhydrous)	0.109	0.109	0.109	0.109	0.109	0.109
Amino Acids
L-Arginine • HCl	0.084	0.084	0.084	0.084	0.084	—
L-Cystine • 2HCl	0.0626	0.0626	0.0626	0.0626	0.0626	0.0626
L-Glutamine	0.584	—	0.584	—	0.584	0.584
Glycine	0.03	0.03	0.03	0.03	0.03	0.03
L-Histidine • HCl • H₂O	0.042	0.042	0.042	0.042	0.042	0.042
L-Isoleucine	0.105	0.105	0.105	0.105	0.105	0.105
L-Leucine	0.105	0.105	0.105	0.105	0.105	—
L-Lysine • HCl	0.146	0.146	0.146	0.146	0.146	—
L-Methionine	0.03	0.03	0.03	0.03	0.03	0.03
L-Phenylalanine	0.066	0.066	0.066	0.066	0.066	0.066
L-Serine	0.042	0.042	0.042	0.042	0.042	0.042
L-Threonine	0.095	0.095	0.095	0.095	0.095	0.095
L-Tryptophan	0.016	0.016	0.016	0.016	0.016	0.016
L-Tyrosine • 2Na • 2H₂O	0.10379	0.10379	0.10379	0.10379	0.10379	0.10379
L-Valine	0.094	0.094	0.094	0.094	0.094	0.094
Vitamins
Choline Chloride	0.004	0.004	0.004	0.004	0.004	0.004
Folic Acid	0.004	0.004	0.004	0.004	0.004	0.004
myo-Inositol	0.0072	0.0072	0.0072	0.0072	0.0072	0.0072
Niacinamide	0.004	0.004	0.004	0.004	0.004	0.004
D-Pantothenic Acid (hemicalcium)	0.004	0.004	0.004	0.004	0.004	0.004
Pyridoxal • HCl	—	—	—	—	0.004	—
Pyridoxine • HCl	0.004	0.004	0.004	0.004	—	0.004
Riboflavin	0.0004	0.0004	0.0004	0.0004	0.0004	0.0004
Thiamine • HCl	0.004	0.004	0.004	0.004	0.004	0.004
Other
D-Glucose	1	4.5	4.5	4.5	4.5	1
HEPES	—	5.958	—	—	—	—
Phenol Red • Na	0.0159	0.0159	0.0159	0.0159	0.0159	—
Pyruvic Acid • Na	0.11	—	0.11	0.11	0.11	—
Add
Glucose	—	—	—	—	—	—
L-Glutamine	—	0.584	—	0.584	—	—
Sodium Bicarbonate	—	—	—	—	3.7	—

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