US EPA Method 632: HPLC Analysis of Pesticides on Ascentis® C18
Materials
analytical column
Product No.
Description
Pricing
Guard cartridge
Product No.
Description
Pricing
Ascentis® C18 Supelguard Guard Cartridge
5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 eaAscentis® C18 Supelguard Guard Cartridge
3 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 eaAscentis® C8 Supelguard Guard Cartridge
5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 eaaccessory
Product No.
Description
Pricing
Wheaton wide-mouth bottle
capacity 32 oz (1000 mL), amber, white polypropylene cap, PTFE flat linerCoupler for Legacy Guard Column Holder
PEEK, I.D. × O.D. × Overall L 0.010 in. × 1/16 in. × 1 in.Supelguard Guard Cartridge Holder
Stand-alone, for use with Supelguard cartridges (2 cm L. x 2.1 to 4.0 mm I.D.)CONDITIONS
column
Ascentis C18, 25 cm x 4.6 mm I.D., 5 μm particles (581325-U)
mobile phase
[A] water; [B] acetonitrile
gradient
10 to 50% B in 13 min; to 60% B in 10 min; to 90% B in 6 min; held for 5 min
flow rate
1.0 mL/min
column temp.
30 °C
detector
UV, 210 nm
injection
10 μL
sample
as indicated in 16% acetonitrile in water
Description
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany