UHPLC Gradient Analysis of Boswellic Acids on Titan C18

column

Titan C18, 10 cm x 2.1 mm I.D., 1.9 μm particles (577124-U)

mobile phase

[A] 0.1% phosphoric acid; [B] acetonitrile

gradient

40% B for 0.5 min; to 100% B in 2 min; held at 100% B for 10 min

flow rate

0.4 mL/min

pressure

4050 psi (279 bar)

column temp.

35 °C

detector

UV, 210 nm

injection

2 μL

sample

6 Boswellic Acids 50μg/mL in 70:30, water:methanol