UHPLC Analysis of Fat Soluble Vitamins on Titan C18, Methanol Mobile Phase

column

Titan C18, 50 mm x 2.1 mm I.D., 1.9 μm particles (577122-U)

mobile phase

100% methanol

flow rate

0.4 mL/min

pressure

2741 psi (189 bar)

column temp.

30 °C

injection

UV, 280 nm

injection

0.5 μL

sample

All compounds at 0.5 mg/mL except retinyl acetate at 0.05 mg/mL in methanol