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MilliporeSigma

UHPLC/MS Analysis of Insulin Analogs on BIOshell A160 Peptide C18

UHPLC/MS Analysis of Insulin Analogs on BIOshell A160 Peptide C18 application for UHPLC-MS

CONDITIONS

column

BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.0 μm particles (67243-U)

mobile phase

[A] 75:25 (0.1% DFA in water: 0.1% DFA in acetonitrile); [B] 50:50 (0.1% DFA in water: 0.1% DFA in acetonitrile)

gradient

23% B for 7 min; 23% B to 100% B in 1 min; hold at 100% B for 7 min

flow rate

0.2 mL/min

pressure

2500 psi (172 bar)

column temp.

75 °C

detector

ESI(+), TIC 100-3000 m/z

injection

0.5 μL

sample

Analog Mixture, 20 μg/mL, 6:3:1 methanol:water:acetic acid containing 0.1% rat plasma

Description

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