TLC Analysis of Water Soluble Vitamins on HPTLC Silica Gel 60 F 254 with Concentrating Zone
Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
TLC pre-coated plate silica gel 60 F 254 with concentrating zone (1.13728.0001, 1.13727.0001)
eluent
[A] chloroform; [B] methanol; (60:40, A:B) 80% saturated with water, containing 01. M lithium chloride
migration distance
6 cm
chamber
normal chamber without chamber saturation
sample
1 μL
detector
in situ evaluation with TLC/HPTLC scanner (Camag) 270 nm