TLC Analysis of Alkaloids on HPTLC Silica Gel 60 F 254
Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
1.05642 HPTLC pre-coated plate silica gel 60 F 254s (1.05642.0001, 1.05548.0001)
eluent
[A] methanol; [B] ammonia 25%; (99:1, A:B)
migration distance
7 cm
chamber
normal chamber without chamber saturation
detector
in situ evaluation with TLC/HPTLC scanner (Camag) UV 254 nm