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LC/MS/MS Analysis of Estradiol (Underivatized) from Patient Serum on Titan C18, 10 cm Column

LC/MS/MS Analysis of Estradiol (Underivatized) from Patient Serum on Titan C18, 10 cm Column application for LC-MS

Materials

standard

Product No.
Description
Pricing

1250008

Estradiol

United States Pharmacopeia (USP) Reference Standard

E-060

17β-Estradiol solution

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

E-073

17β-Estradiol-2,3,4-13C3 solution

ampule of 1 mL, Cerilliant®

CONDITIONS

column

Titan C18, 10 cm x 2.1 mm I.D., 1.9 μm particles (577124-U)

mobile phase

[A] 0.2 mM ammonium fluoride; [B] methanol

gradient

40 to 82%B in 4.6 min; to 100%B in 0.05 min; held for 1.15 min; to 40% B in 0.05 min; held for 1.65 min

flow rate

0.4 mL/min

column temp.

40 °C

detector

MS, ESI(-), MRM transitions listed on figure

injection

20 μL

sample

patient serum sample containing estradiol, 56 pmol/L

Description

Analysis Note

A fast and robust LC/MS/MS method was developed for the measurement of estradiol in human serum using Titan C18 1.9 μm columns. Ultra high purity LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant certified reference material grade standards provided reliable quantification.

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