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LC/MS Analysis of Antipsychotic Drugs on Ascentis® Express RP Amide

LC/MS Analysis of Antipsychotic Drugs on Ascentis® Express RP Amide application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Express RP-Amide, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

Standard

Product No.
Description
Pricing

Bupropion hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

(±)-Hydroxybupropion solution

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

9-Hydroxyrisperidone solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express RP Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U)

mobile phase

[A] 5 mM ammonium formate water; [B] 5mM ammonium formate in 95:5 (v/v) methanol:water

gradient

held at 35% B for 0.5 min; 35 to 85% B in 5.5 min; held at 85% B for 1 min

flow rate

0.3 mL/min

pressure

4641 psi (320 bar)

column temp.

35 °C

detector

ESI(+), m/z 100-1000

injection

1 μL

sample

200 ng/mL in waler

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany