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HPLC Size-Exlusion Analysis of mAb Monomer, Dimer, and Higher-Order Aggregates on TSKgel® SuperSW mAb HTP

HPLC Size-Exlusion Analysis of mAb Monomer, Dimer, and Higher-Order Aggregates on TSKgel® SuperSW mAb HTP application for HPLC

Materials

Analytical column

Product No.
Description
Pricing

822855

TSKgel® SuperSW mAb HTP HPLC Column

phase diol, L × I.D. 15 cm × 4.6 mm, 4 μm particle size

guard column

Product No.
Description
Pricing

822858

TSKgel® SuperSW mAb HTP Guard Column

phase diol, L × I.D. 2 cm × 3 mm, 4 μm particle size

CONDITIONS

column

TSKgel SuperSW mAb HTP 4 μm, 15 cm x 4.6 mm I.D., 4 μm particles (822855)

mobile phase

0.2 M phosphate buffer, pH 6.7 + 0.05% sodium azide

flow rate

0.5 mL/min and 0.35 mL/min

column temp.

25 °C

detector

UV, 280 nm

injection

5 μL

sample

Monoclonal antibody 2 (mouse-human chimeric IfG, Erbitux)

Description

Analysis Note

Size-exclusion chromatography is now routinely used in the quality control of commercial monoclonal antibody (mAb) preparations. In this example, a mAb monomer, dimer and higher order aggregates are easily separated within 5 minutes on a 15 cm x 4.6 mm ID TSKgel® SuperSW mAb HTP column at a high flow rate of 0.35 mL/min. After Increasing the flow rate to 0.5 mL/min the monomer elutes within 3 minutes from the column.

Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS