HPLC-DAD Analysis of Metformin Hydrochloride on Ascentis® Express C18 HPLC Column
Materials
analytical column
mobile phase component
Milli-Q® IQ 7000 Ultrapure Water Purification System
output: type 1 water (18.2 MΩ·cm), the most advanced Milli-Q® ultrapure (Type 1) water purification system that is intelligent, intuitive, and green.standard
CONDITIONS
column
Ascentis® C18, 25 cm × 4.6 mm I.D., 5 μm particle size (581325-U)
mobile phase
[A] 20 mM Potassium hexafluorophosphate in water; [B] Methanol
gradient
95%A to 55%A in 8min, 8-10 min 55%A, 10-16 min 95%A
flow rate
1.0 mL/min
column temp.
25 °C
detector
(DAD, wavelength: 230 nm)
injection
(5 µL)
sample/matrix
0.1 mg/mL in water (PHR1084)
Description
General description
Metformin is a biguanide hypoglycemic agent and an oral antidiabetic medication widely used in the treatment of type 2 diabetes. It is effective in glycemic control and decreases the intestinal absorption of glucose, without the problem of serious lactic acidosis.
Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards. They are provided with a comprehensive Certificate of Analysis (COA) included a certified value and expanded uncertainty according to ISO 17034. Additionally, traceability to the current batch of Compendial Reference Standards from USP, EDQM and/or BP is provided, as available.
sigmaaldrich.com/pharmastandards
Other Notes
APP_788
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
