HPLC Analysis of Ulcer Treatment Drugs on Ascentis^® RP-Amide

column

Ascentis RP-Amide, 15 cm x 2.1 mm I.D., 5 μm particles (565305-U)

mobile phase

[A] 10 mM ammonium phosphate, pH 6.2 with phosphoric acid; [B] acetonitrile

gradient

5 to 15% B in 10 min; to 55% B in 8 min; held for 5 min

flow rate

0.2 mL/min

column temp.

35 °C

detector

UV, 220 nm

injection

5 μL

sample

as indicated in 95:5 water:methanol