HPLC Analysis of Proteins (2) on BIOshell A400 Protein C4
Materials
analytical column
Product No.
Description
Pricing
CONDITIONS
sample
protein mixture, g/L ea: superoxide dismutase, 0.3; bovine serum albumin, 0.3; alpha-lactalbumin, 0.2; beta-lactoglobulin A, 0.3; catalase, 0.3; (carbonic anhydrase, 0.3; beta-Amylase, 1.0; glucose-6-phosphate dehydrogenase, 1.0; thioredoxin, 0.3; phosphorylase b, 2.5.)
column
BIOshell A400 Protein C4, 10 cm X 2.1 mm I.D., 3.4 μm particles (66825-U)
mobile phase
[A] 75:25, (0.1% TFA in water):(0.1% TFA in acetonitrile) ; [B] 25:75, (0.1% TFA in water):(0.1% TFA in acetonitrile)
gradient
15 to 75% B in 10 min
flow rate
0.3 mL/min
pressure
2410 psi (166 bar)
column temp.
30 °C
detector
UV, 215 nm
injection
1 μL
Description
Analysis Note
This application demonstrates the utility of the BIOshell A400 Protein C4 column for the reversed-phase chromatography of proteins from a variety of sources and of different molecular weights.
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