HPLC Analysis of Fluoroquinolone Antibiotics on Ascentis® Express C18
Materials
Analytical column
Product No.
Description
Pricing
CONDITIONS
column
Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)
mobile phase
[A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile; (85:15, A:B)
flow rate
1.2 mL/min
column temp.
35 °C
detector
UV, 254 nm
injection
4 μL
sample
200 ng/mL in 25:75, water: acetonitrile
Description
Analysis Note
This application demonstrates the suitability of the Ascentis Express C18 for the analysis of fluoroquinolones.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany