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HPLC Analysis of Erythropoietin on SRT® SEC-300 Gel Filtration Column

HPLC Analysis of Erythropoietin on SRT® SEC-300 Gel Filtration Column application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Z777051

SRT® SEC-300 Gel Filtration Column, 5 μm

L × I.D. 30 cm × 7.8 mm, 5 μm particle size, stainless steel

mobile phase component

Product No.
Description
Pricing

255793

Sodium phosphate dibasic

99.95% trace metals basis

CONDITIONS

column

SRT SEC-300, 30 cm x 7.8 mm I.D., 5 μm partices, 300 Å, (Z777051)

mobile phase

Na2HPO4-KH2PO4-NaCl

flow rate

0.7 mL/min

column temp.

Ambient

detector

UV, 280 nm

injection

20 μL

sample

EPO (Recombinant Human Erythropoietin)

Description

Analysis Note

Erythropoietin (EPO) is a glycoprotein hormone that controls red blood cell production. EPO was one of the first genetically engineered therapeutic proteins. It is available under such brand names as Epogen and Aranesp for the treatment of anemia. Human EPO has a molecular mass of 34 kDa. The 5 micron SRT SEC-300 column is operated at a flow rate of 0.7 mL/min. Note that after the main peak, an impurity elutes at about 16 minutes.

Legal Information

SRT is a registered trademark of Sepax Technologies

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