HPLC Analysis of E. coli Lysate on Proteomix^® SAX-NP3, Demonstration of High Loading Capacity

column

Proteomix SAX-NP3, 5 cm x 4.6mm I.D., 3 μm particles (Z777219)

mobile phase

(A) 20 mM Tris, pH 9.0; (B) A + 0.5 M NaCl

gradient

0 - 100% B in 30 min

flow rate

0.5 mL/min

column temp.

25 °C

detector

UV, 280 nm

injection

as indicated in figure

sample

2.5 mg/mL