HPLC Analysis of Diclofenac and Salicylic Acid on Ascentis^® RP-Amide

column

Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)

mobile phase

[A] 20 mM phosphoric acid (pH 2.0 unadjusted); [B] acetonitrile; (50:50, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 220 nm

injection

10 μL

sample

as indicated in 20 mM phosphoric acid (pH 2.0)