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MilliporeSigma

HPLC Analysis of Caffeine and Theophylline on SeQuant® ZIC-HILIC

HPLC Analysis of Caffeine and Theophylline on SeQuant® ZIC-HILIC application for HPLC

Materials

accessory

Product No.
Description
Pricing

Bottle-type sprayer

capacity 240 mL

Rectangular TLC developing tanks, complete

L × H × W 27.0 cm × 26.5 cm × 7.0 cm

Sigma TLC spray box

Spectroline® E-Series UV lamp

output8 W, AC/DC input 115 V AC

Rectangular TLC developing tanks, complete

L × H × W 7.5 cm × 15.5 cm × 8.0 cm

Hamilton® TLC syringes

1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),

coated tip

Spectroline® E-Series UV lamp

output6 W, AC/DC input 120 V AC

Hamilton® TLC syringes

1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),

coated tip

Hamilton® TLC syringes

1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),

coated tip

Rectangular TLC developing tanks, complete

L × H × W 17.5 cm × 16.0 cm × 8.2 cm

Spectroline® E-Series UV lamp

output6 W, AC/DC input 230 V AC

Spectroline® E-Series UV lamp

output4 W, AC/DC input 230 V AC

Spectroline® E-Series UV lamp

output8 W, AC/DC input 230 V AC

Latch-lid TLC developing chambers

For use with 10 cm × 10 cm plates

Hamilton® TLC syringes

1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),

coated tip

Latch-lid TLC developing chambers

For use with 20 cm × 20 cm plates

Spectroline® E-Series UV lamp

output4 W, AC/DC input 115 V AC

Kimble® microcapillary pipettes

Kimble® microcapillary pipettes

Kimble® microcapillary pipettes

capacity 20 μL, accuracy: ±0.5%

Reagent sprayer for TLC plates

volume 250 mL, Bottle-Type

CONDITIONS

column

1.50448 Sequant ZIC-HILIC, PEEK, 150 x 4.6 mm, 5 μm, 200 Å (1.50448.0001)

mobile phase

[A] acetonitrile; [B] ammonium acetate; (90:10, A,B)

flow rate

0.1 mL/min

pressure

403 psi (2.8 MPa)

detector

254 nm

injection

5 μL

Description

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany