HPLC Analysis of Bupivacaine Enantiomers on Astec^® CHIROBIOTIC^® V

column

CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)

mobile phase

[A] 1% triethylamine, pH 4.1; [B] methanol; (5:95, A:B)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 202 nm

injection

1 μL

sample

1 mg/mL in methanol