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HPLC Analysis of Biogenic Amines on Ascentis® Si, HILIC Mode, (90 % Acetonitrile)

HPLC Analysis of Biogenic Amines on Ascentis® Si, HILIC Mode, (90 % Acetonitrile) application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Si HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

CONDITIONS

column

Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)

mobile phase

[A] 0.1% ammonium acetate in water (pH unadjusted); [B] 0.1% ammonium acetate in acetonitrile; (10:90, A:B)

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 280 nm

injection

10 μL

sample

50 ug/mL each in 0.1% ammonium acetate in 10:90:water:acetonitrile

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany