HPLC Analysis of Anti-Inflammatory Drugs on Discovery^® RP-Amide C16

column

Discovery RP-Amide C16, 15 cm × 4.6 mm I.D., 5 μm particles (505013)

mobile phase

(A) acetonitrile; (B) 25 mM KH₂PO₄, pH 3.0; (40:60, A:B)

flow rate

1 mL/min

column temp.

30 °C

detector

UV, 230 nm

injection

10 μL, 1 μg/mL each analyte