HPLC Analysis of Angiotensins on Discovery^® C18

column

Discovery C18, 15 cm × 4.6 mm I.D., 5 μm particles (504955)

mobile phase

(A) 5 mM (NH₄)H₂PO₄/NH₄OH, pH 7.0; (B) 5 mM (NH₄)H₂PO₄/NH₄OH, pH 7.0: CH₃CN (50:50, A:B)

gradient

30-60% B in 15 min

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 220 nm