HPLC Analysis of Biogenic Amines on Ascentis® Si, HILIC Mode (85 % Acetonitrile)
Materials
Analytical column
Product No.
Description
Pricing
CONDITIONS
column
Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)
mobile phase
[A] 0.1% ammonium acetate in water (pH unadjusted); [B] 0.1% ammonium acetate in acetonitrile; (15:85, A:B)
flow rate
1 mL/min
column temp.
35 °C
detector
UV, 280 nm
injection
10 μL
sample
50 μg/mL in 0.1% ammonium acetate in 10:90:water:acetonitrile
Description
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany