GC Analysis of Stored Apples on 5% Phenyl Column after SPME using 100 μm PDMS Fiber
Materials
analytical column
SPME fiber
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB)
df 65 μm(PDMS/DVB, needle size 24 ga, for use with manual holderrelated product
CONDITIONS
sample/matrix
300-450g intact fruit in a 3 liter flask at ambient temp, exposed to an air flow of 25-30 mL/min
SPME fiber
polydimethylsiloxane, 100 μm film (57310-U)
desorption process
250°C for 90 sec
column
5% phenyl polysiloxane, 25m × 0.25 mm I.D., 0.34 μm film (Supelco equivalent, SLB-5ms, 28473-U)
oven
40 °C (1.5 min) to 250 °C at 50 °C/min, hold 1 min
inj. temp.
250 °C
detector
mass spectrometer, m/z = 40-300 (40 spectra/sec)
carrier gas
helium, 1.5 mL/min (44 cm/min)
sample
Apples
Description
Analysis Note
SPME can reduce sampling time and is complementary to rapid separation/detection techniques such as time compressed gas chromatography (TCGC) and time-of-flight mass spectrometry (TOF-MS), to permit analysis of dozens of compounds in a few minutes. Investigators have used SPME to monitor flavor volatiles in apple, strawberry, tomato, and many other fruits and vegetables. In this application, 65 μm PDMS/divinylbenzene (DVB) SPME fibers were used to capture volatiles emanating from Mutsu apples stored for 3-4 months at 0 °C.