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Endothelial-like nitric oxide synthase immunolocalization by using gold nanoparticles and dyes.

Endothelial-like nitric oxide synthase immunolocalization by using gold nanoparticles and dyes.

Biomedical optics express (2015-12-30)

Ramla Gary, Daniela Amelio, Filippo Garofalo, Gia Petriashvili, Maria Penelope De Santo, Yuen Kwong Ip, Riccardo Barberi

ABSTRACT

Immunofluorescence is a biological technique that allows displaying the localization of the target molecule through a fluorescent microscope. We used a combination of gold nanoparticles and the fluorescein isothiocianate, FITC, as optical contrast agents for laser scanning confocal microscopy imaging to localize the endothelial-like nitric oxide synthase in skeletal muscle cells in a three-dimensional tissue phantom at the depth of 4µm. The FITC detected fluorescence intensity from gold-nanoparticles-labelled cells was brighter than the emission intensity from unlabelled cells.

MATERIALS

Product Number

Brand

Product Description

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