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Signature microRNAs in human cornea limbal epithelium.

Functional & integrative genomics (2014-12-10)
Yufei Teng, Hoi Kin Wong, Vishal Jhanji, Jian Huan Chen, Alvin Lerrmann Young, Mingzhi Zhang, Kwong Wai Choy, Jodhbir Singh Mehta, Chi Pui Pang, Gary Hin-Fai Yam
ABSTRACT

This study was aimed to identify the signature microRNAs, which regulate the biological processes of corneal epithelial progenitor cell (CEPC) homeostasis and regulation through characterizing the differential expression profile of microRNAs in human limbal epithelium containing adult CEPC versus central corneal epithelium without CEPC. MicroRNA microarray had identified 37 microRNAs enriched in human corneal epithelium. Among them, nine were significantly upregulated in limbal epithelium and one in central corneal epithelium after validation by TaqMan® real-time polymerase chain reaction. In addition to our previous finding of miR-143 and 145, the expression of miR-10b, 126, and 155 was localized in limbal epithelium (LE) (predominantly basal layers) by using locked nucleic acid-based in situ hybridization. Potential target genes were predicted by TargetScan Human v6.0 and compared to the reported human cornea epithelial gene profile GSE5543. Analyzed by web-based Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and DAVID Functional Annotation Bioinformatics Resources v6.7, the downregulated genes were involved in pathways of immune response and cellular protection, apoptosis, and cell movement whereas upregulated genes with cell survival, cell-matrix interaction, and cell-cell adhesion. We found a constant occurrence of miR-143, 145, and 155 in all KEGG pathways regulating limbal epithelial events. By Ingenuity Systems (IPA®) analysis, these microRNAs could cooperatively regulate cell growth and apoptosis via tumor necrosis factor activation and MYC repression. Our findings thus suggest a unique microRNA signature existing in human limbal epithelium and participating in CEPC homeostasis.

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Chloroform, suitable for HPLC
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Chloroform, ACS reagent, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
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Chloroform, SAJ super special grade, ≥99.0%
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Chloroform, SAJ first grade, ≥99.0%, contains 0.4-0.8% ethanol
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Chloroform, JIS 300, ≥99.0%, for residue analysis
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Chloroform, JIS special grade, ≥99.0%
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Chloroform, ≥99%, PCR Reagent, contains amylenes as stabilizer
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Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%
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Chloroform, analytical standard
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Chloroform, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer
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Chloroform, suitable for HPLC, ≥99.8%, amylene stabilized
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Chloroform, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
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Chloroform, puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)
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Chloroform, contains ethanol as stabilizer, meets analytical specification of BP, 99-99.4% (GC)
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Chloroform, Pharmaceutical Secondary Standard; Certified Reference Material
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Isopropyl alcohol, ≥99.7%, FCC, FG
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2-Phenylindole, technical grade, 95%
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MXP 19,563.00